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- Title
Quantum Molecular Resonance Inhibits NLRP3 Inflammasome/Nitrosative Stress and Promotes M1 to M2 Macrophage Polarization: Potential Therapeutic Effect in Osteoarthritis Model In Vitro.
- Authors
Paolucci, Teresa; Pino, Vanessa; Elsallabi, Osama; Gallorini, Marialucia; Pozzato, Gianantonio; Pozzato, Alessandro; Lanuti, Paola; Reis, Victor Machado; Pesce, Mirko; Pantalone, Andrea; Buda, Roberto; Patruno, Antonia
- Abstract
This study aimed to investigate the anti-inflammatory effects of Quantum Molecular Resonance (QMR) technology in an in vitro model of osteoarthritis-related inflammation. The study used THP-1-derived macrophages stimulated with lipopolysaccharide and hyaluronic acid fragments to induce the expression of inflammatory cytokines and nitrosative stress. QMR treatment inhibited COX-2 and iNOS protein expression and activity and reduced NF-κB activity. Furthermore, QMR treatment led to a significant reduction in peroxynitrite levels, reactive nitrogen species that can form during inflammatory conditions, and restored tyrosine nitration values to those similar to sham-exposed control cells. We also investigated the effect of QMR treatment on inflammasome activation and macrophage polarization in THP-1-derived macrophages. Results showed that QMR treatment significantly decreased NLRP3 and activated caspase-1 protein expression levels and downregulated IL-18 and IL-1β protein expression and secretion. Finally, our findings indicate that QMR treatment induces a switch in macrophage polarization from the M1 phenotype to the M2 phenotype.
- Subjects
NLRP3 protein; TREATMENT effectiveness; REACTIVE nitrogen species; INFLAMMASOMES; MACROPHAGES
- Publication
Antioxidants, 2023, Vol 12, Issue 7, p1358
- ISSN
2076-3921
- Publication type
Article
- DOI
10.3390/antiox12071358