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- Title
Effects of Gossypol Acetic Acid on DNA Methylation Level and mRNA Expression of p16 Gene in Human Adenoid Cystic Carcinoma ACC-MCells.
- Authors
YUE Wan-yuan; CHEN Wen-fei; FU Shuai; WU Yong
- Abstract
Objective To study the effects of gossypol acetic acid (GAA) on methylation level and mRNA expression of p16 gene in human adenoid cystic carcinoma ACC-M cells in vitro, and investigate the antitumor mechanism of GAA. Methods (1) Methylation specific PCR (MS-PCR) was used to detect methylation level changing of the p16 in ACC-M cells treated by GAA for 48 h and 72 h. (2) Real-time fluorescence quantitative PCR(RFQ-PCR) was used to detect mRNA expression level changing of the p16 in ACC-M cells treated by GAA for 48 h and 72 h. Results (1) Compared with the control group, MS-PCR showed the hypermethylation of CpG island in gene promoter of p16 was partly reversed after GAA treatment(19 μ mol/L GAA for 48 h and 10 μmol/L for 72 h, P< 0.05) . (2) The results of RFQ-PCR showed that mRNA expression of p16 in ACC-M cells was significantly higher than that in the control group after GAA treatment (19 μmol/L GAA for 48 h and 10 μmol/L for 72 h, P< 0.05) . Conclusion GAA can reduce the p16 gene methylation level and increase p16 gene mRNA expression, which might be one of the mechanisms of GAA antitumor activity.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2014, Vol 35, Issue 5, p17
- ISSN
2095-610X
- Publication type
Article