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- Title
Advenella kashmirensis subsp. methylica PK1, a facultative methylotroph from carex rhizosphere.
- Authors
Poroshina, M.; Doronina, N.; Kaparullina, E.; Trotsenko, Yu.
- Abstract
The strain (PK1) of facultative methylobacteria growing on methanol as a carbon and energy source was isolated from carex rhizosphere (Pamukkale National Park, Turkey). The cells were nonmotile gram-negative rods propagating by binary fission. The organism was a strict aerobe, oxidase- and catalasepositive. Optimal growth occurred at 29°C, pH 8.0-8.5, and 0.5% NaCl; no growth occurred at 2% NaCl. The organism used the ribulose bisphosphate pathway of C assimilation. Predominant fatty acids were 11-octodecenoic (18:1ω7) and cis-hexadecenoic (16:1ω7c). Phosphatidylethanolamine and diphosphatidylglycerol were the dominant phospholipids. Q8 was the main ubiquinone. The DNA G+C content was 55.4 mol % mp. Sequencing of the 16S rRNA gene revealed that strain PK1 belonged to the genus Advenella with 98.8 and 99.2% similarity to the type strains A. incenata CCUG 45225 and A. kashmirensis WT001, respectively. DNA-DNA homology of strain PK1 and A. kashmirensis WT001 was 70%. While the MALDI analysis confirmed their close clusterization, RAPD analysis revealed the differences between strain PK1 and other Advenella strains. Based on its geno- and phenotypic properties, the isolate PK1 was classified as A. kashmirensis subsp. methylica PK1 (VKM-B 2850 = DSM 27514), the first known methylotroph of the genus Advenella.
- Subjects
METHYLOBACTERIUM; BACTERIAL cultures; METHYLOTROPHIC microorganisms; BACTERIAL growth; ISOLATION of biotechnological microorganisms; RHIZOBACTERIA; CAREX
- Publication
Microbiology (00262617), 2015, Vol 84, Issue 1, p73
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261715010117