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- Title
Inductive Effect of Hypoxanthine–Xanthine Oxidase System on Lambda Prophage.
- Authors
Zhu, S. Q.; Fang, C. X.; Zhu, S. H.; Zhang, L. Z.; Fan, C. P.; Zhang, J. X.
- Abstract
Hypoxanthine–xanthine oxidase (HX–XO) system is a classical system that can generate superoxide anions. The inductive effect of the HX–XO system for lambda prophage was investigated in this study. The results showed that the system can induce lambda prophage from the lysogenic state to lytic growth. The inductive effect was directly proportional to the concentration of HX and XO and inversely related to the time of preliminary incubation of HX with XO.The cell density of the lysogenic bacteria also greatly affected the inductive effect. The maximal PFU number of 2.9 × 104 PFU/ml was recorded at 0.86 mM HX, 1.6 × 10–2 U/ml XO, and a cell density of 108 cells/ml. The inductive effect of the HX–XO system was inhibited in the suspensions by glutathione, superoxide dismutase, and catalase. The results provide evidence that free radicals are the primary factors in the induction of lambda prophage in lysogenic bacteria.
- Subjects
ANIONS; IONS; CELLS; GLUTATHIONE; FREE radical reactions; CHEMICAL reactions
- Publication
Microbiology (00262617), 2004, Vol 73, Issue 1, p42
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1023/B:MICI.0000016366.98529.b7