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- Title
Long noncoding RNA OIP5-AS1 exhibits oncogenic activity in bladder cancer through miR-217 and MTDH.
- Authors
ZHANG, S.-F.; PANG, S.; WANG, F.-P.; LI, X.-Y.; ZOU, Q.
- Abstract
OBJECTIVE: The aim of this study was to investigate the role of long non-coding Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) in bladder cancer (BCa), and the mechanism of OIP5-AS1/microRNA-217 (miR- 217)/metadherin (MTDH) in promoting the progression of BCa. PATIENTS AND METHODS: OIP5-AS1, miR- 217 and MTDH expressions in BCa tissues and cells were detected by qRT-PCR or Western blot. CCK-8 and transwell assays were used to determine the proliferation and invasion of BCa cells. The correlation between OIP5-AS1 and miR-217, miR-217 and MTDH, and OIP5-AS1 and MTDH were studied by Luciferase reporter assay and Spearman correlation analysis. Statistical analysis of test data was performed using t-test. RESULTS: OIP5-AS1 was upregulated in BCa tissues and cells, and OIP5-AS1 knockdown could inhibit the proliferation and invasion of BCa cells. MiR-217 was a direct-acting target of OIP5- AS1, and MTDH was a target of miR-217. OIP5- AS1 knockdown inhibits human BCa cell proliferation and invasion through miR-217/MTDH axis. CONCLUSIONS: This study systematically explored the effect of OIP5-AS1 in human BCa. MiR-217/MTDH pathway mediated the promotion of OIP5-AS1 in BCa cells proliferation and invasion. OIP5-AS1, as an oncogene, could be used as a biomarker for the treatment of BCa.
- Subjects
LINCRNA; BLADDER cancer; ANTISENSE RNA; ANTISENSE peptides; RANK correlation (Statistics); ONCOGENES
- Publication
European Review for Medical & Pharmacological Sciences, 2021, Vol 25, Issue 8, p3211
- ISSN
1128-3602
- Publication type
Article