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- Title
A study of phytochrome-membrane association using an enzymelinked immunosorbent assay and Western blotting.
- Authors
Jordan, Brian R.; Partis, Michael D.; Thomas, Brian
- Abstract
Enzyme-linked immunosorbent assay (ELISA) and Western blotting have been used to study the association between phytochrome and membranes in dark-grown tissue of Avena sativa L. cv. Saladin. Enhanced phytochrome pelletability can be demonstrated by ELISA after red irradiation in vivo and extraction in buffer containing Mg2+, but not with red irradiation of Mg2+ alone. This enhanced association may, however, be restricted to specific membranes. The results obtained with ELISA are confirmed by Western blotting techniques. Membrane-associated and soluble phytochrome give different immunological responses and it is suggested that immunologically distinct populations of phytochrome may exist in dark-grown tissue.
- Subjects
OATS; ENZYME-linked immunosorbent assay; MAGNESIUM; WESTERN immunoblotting; PHYTOCHROMES; IMMUNOENZYME technique
- Publication
Physiologia Plantarum, 1984, Vol 60, Issue 3, p416
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1984.tb06085.x