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- Title
Effects of long-term intravenous and intragastricl-arginine intervention on jejunal motility and visceral nitric oxide production in the hyperdynamic compensated endotoxaemic pig.
- Authors
Bruins, M. J.; Luiking, Y. C.; Soeters, P. B.; Lamers, W. H.; Akkermans, L. M. A.; Deutz, N. E. P.
- Abstract
Alterations inl-arginine availability and nitric oxide (NO) synthesis in the intestinal muscularis may contribute to disturbed small intestinal motility that is observed during endotoxaemia. The aim of this study was to evaluate the effect ofl-arginine infusion on visceral NO production and jejunal motility in hyperdynamic compensated endotoxaemic pigs. Fasted and saline-resuscitated pigs were intravenously infused for 24 h with endotoxin (lipopolysaccharide, 50 ng kg−1 min−1) or saline (n = 6). Endotoxaemic pigs received either intravenousl-arginine (n = 6, 5.3 μmol kg−1 min−1) orl-alanine (isocaloric,n = 6). After 24 h, intravenousl-arginine orl-alanine infusion was continued intragastrically for 32-h in an enteral meal. During (0–24 h) and 1 day postendotoxaemia (48–56 h), jejunal motility was recorded by manometry and analysed for migrating motor complex (MMC) characteristics. Visceral NO production was measured at 24 and 48 h by15 N2-arginine-to-15 N-citrulline conversion. Visceral NO production was increased during endotoxaemia and was higher inl-arginine than inl-alanine-treated pigs. One day postendotoxaemia, visceral NO synthesis was still increased inl-arginine but not inl-alanine-treated animals. Endotoxaemia shortened the MMC cycle duration and accelerated the MMC propagation velocity. Both were restored byl-arginine. Similar motility disturbances were observed one day postendotoxaemia and were also compensated byl-arginine infusion.
- Subjects
ARGININE; NITRIC oxide; ENDOTOXINS; GASTROINTESTINAL motility; NEUROTRANSMITTERS
- Publication
Neurogastroenterology & Motility, 2004, Vol 16, Issue 6, p819
- ISSN
1350-1925
- Publication type
Article
- DOI
10.1111/j.1365-2982.2004.00579.x