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- Title
Buspirone-induced antinociception is mediated by l-type calcium channels and calcium/caffeine-sensitive pools in mice.
- Authors
Liang, Jian-Hui; Wang, Xu-Hua; Liu, Rui-Ke; Sun, Hong-Lei; Ye, Xiang-Feng; Zheng, Ji-Wang
- Abstract
Rationale: Previous studies have shown that buspirone, a partial 5-HT)[SUB1A] receptor agonist, produces antinociceptive effects in rats and mice; Ca[SUP2+] plays a critical role as a second messenger in mediating nociceptive transmission. 5-HT[SUB1A] receptors have been proven to be coupled functionally with various types of Ca[SUP2+] channels in neurons, including N-, P/Q-, T-, or L-type. It was of interest to investigate the involvement of extracellular/intracellular Ca[SUP2+] in buspirone-induced antinociception. Objectives: To determine whether central serotonergic pathways participate in the antinociceptive processes of buspirone, and investigate the involvement of Ca[SUP2+] mechanisms, particularly L-voltage-gated Ca[SUP2+] channels and Ca[SUP2+]/caffeine-sensitive pools, in buspirone-induced antinociception. Methods: Antinociception was assessed using the hot-plate test (55°C, hind-paw licking latency) in mice treated with either buspirone (1.25-20 mg/kg i.p.) alone or the combination of buspirone and fluoxetine (2.5-10 mg/kg i.p.), 5-HTP (25 mg/kg i.p.), nimodipine (2.5-10 mg/kg i.p.), nifedipine (2.5-10 mg/kg i.p.), CaCl[SUB2] (25-200 nmol per mouse i.c.v.), EGTA (5-30 nmol per mouse i.c.v.), or ryanodine (0.25-2 nmol per mouse i.c.v.). Results: Buspirone dose dependently increased the licking latency in the hot-plate test in mice. This effect of buspirone was enhanced by fluoxetine, 5-HTP, nimodipine, and nifedipine. Interestingly, central administration of Ca[SUP2+] reversed the antinociceptive effects of buspirone. In contrast to these, ryanodine or EGTA administered centrally potentiated buspirone-induced antinociception. Conclusions Decreasing neuronal Ca[SUP2+] levels potentiated buspirone-induced antinociception; conversely, increasing intracellular Ca[SUP2+] abolished the antinociceptive effects of buspirone. These results suggest that Ca[SUP2+] influx from extracellular fluid and release of Ca[SUP2+] from Ca[SUP2+]/caffeine-sensitive...
- Subjects
BUSPIRONE; FLUOXETINE; RYANODINE; CALCIUM antagonists
- Publication
Psychopharmacology, 2003, Vol 166, Issue 3, p276
- ISSN
0033-3158
- Publication type
Article
- DOI
10.1007/s00213-002-1327-4