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- Title
Fatty acids profiling of avocado seed and pulp using gas chromatography-mass spectrometry combined with multivariate chemometric techniques.
- Authors
Aliakbarzadeh, Ghazaleh; Sereshti, Hassan; Parastar, Hadi
- Abstract
In the present study, the profiling of 17 fatty acids (FAs) in avocado seed and pulp was investigated. The fatty acids were extracted with vortex-assisted extraction, methyl esterified and finally preconcentrated by dispersive liquid-liquid microextraction. The preconcentrated fatty acid methyl esters (FAMEs) were analyzed using gas chromatography-mass spectrometry (GC-MS) to obtain qualitative and quantitative information. The GC-MS data were analyzed using multivariate curve resolution-alternating least squares (MCR-ALS) method to overcome general chromatographic problems such as overlapped peaks, background interference and peak shifts. The calibration data were prepared using pure analytical information obtained by MCR-ALS. The linear dynamic ranges and regression coefficients ( R ) for FAMEs were in the range of 0.19-65 mg L and 0.990-0.999, respectively. The relative standard deviation (RSD%) for determination of FAs in avocado seed and pulp was 0.17-8.84 and 0.64-17.93, respectively. The main FAs in the avocado pulp were: oleic acid (74.25 g Kg), linoleic acid (26.87 g Kg), palmitic acid (26.02 g Kg), palmitoleic acid (1.22 g Kg) and stearic acid (0.05 g Kg). And, the main FAs in the avocado seed were: linoleic acid (1.09 g Kg), palmitic acid (0.47 g Kg), oleic acid (0.33 g Kg), linolenic acid (0.12 g Kg), and palmitoleic acid (0.04 g Kg).
- Subjects
FATTY acid methyl esters; AVOCADO; GAS chromatography/Mass spectrometry (GC-MS); EXTRACTION techniques; OLEIC acid
- Publication
Journal of the Iranian Chemical Society, 2016, Vol 13, Issue 10, p1905
- ISSN
1735-207X
- Publication type
Article
- DOI
10.1007/s13738-016-0907-x