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- Title
Single-molecule observation of protein?protein interactions in the chaperonin system.
- Authors
Taguchi, Hideki; Ueno, Taro; Tadakuma, Hisashi; Yoshida, Masasuke; Funatsu, Takashi
- Abstract
We have analyzed the dynamics of the chaperonin (GroEL)—cochaperonin (GroES) interaction at the singlemolecule level. In the presence of ATP and non-native protein, binding of GroES to the immobilized GroEL occurred at a rate that is consistent with bulk kinetics measurements. However, the release of GroES from GroEL occurred after a lag period (∼3 s) that was not recognized in earlier bulk-phase studies. This observation suggests a new kinetic intermediate in the GroEL-GroES reaction pathway.
- Subjects
MOLECULAR chaperones; BIOTECHNOLOGY
- Publication
Nature Biotechnology, 2001, Vol 19, Issue 9, p861
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt0901-861