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- Title
SUCROSE PHOSPHATE SYNTHASE GENE EXPRESSION IN SORGHUM, SWEET SORGHUM AND F5 HYBRID POPULATIONS.
- Authors
JI, Y.; ZHANG, W. T.; PANG, H. B.; LI, X. M.; MA, L. J.; WANG, L. L.; ZHANG, Y.; HUSSAIN, K.; WU, S. W.; LI, Y. Y.
- Abstract
Sweet sorghum has drought resistance, waterlogging resistance, salt and alkali resistance, wide adaptability and excellent resistance to leaf diseases, and is one of China's important food crops, sugar crops and energy crops. In the face of the energy crisis, as an energy crop, sweet sorghum has a high conversion rate of ethanol, which shows an attractive prospect for development and utilization and has attracted the attention of many countries. One of the main indicators to measure the use value of sweet sorghum is its high sugar content, and it is of great significance to increase the sugar content. In this study, we examined the expression of sucrose phosphate synthase (SPS) in leaves of sweet sorghum and sorghum and found the rules of sugar accumulation in sweet sorghum. The effect of SPS gene expression on sucrose accumulation in sweet sorghum and the factors affecting the difference of sugar content between sweet sorghum and sorghum cultivars were studied. The sweet sorghum LTR102 (as the female in this experiment), and common grain sorghum 654 (as the male), and hybrid F5 high and low sugar individuals were used as experimental materials. The gene expression of sucrose phosphate synthase was detected by real-time PCR. According to the qualitative and quantitative analyses of SPS gene, the SPS gene is not only the key factor for the accumulation of sucrose, but also the factor that causes the difference in sugar content between sweet sorghum and common sorghum. At different growth stages, the high sugar individuals showed superiority in sucrose accumulation, SPS activity and SPS gene expression compared to the other three individuals.
- Subjects
GENE expression; SORGHUM; ENERGY crops; LEAF diseases &; pests; ALTERNATIVE grains; MOLECULAR genetics
- Publication
Applied Ecology & Environmental Research, 2018, Vol 16, Issue 5, p6731
- ISSN
1589-1623
- Publication type
Article
- DOI
10.15666/aeer/1605_67316740