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- Title
Machine learning approach for recognition and morphological analysis of isolated astrocytes in phase contrast microscopy.
- Authors
Yakovlev, Egor V.; Simkin, Ivan V.; Shirokova, Anastasiya A.; Kolotieva, Nataliya A.; Novikova, Svetlana V.; Nasyrov, Artur D.; Denisenko, Ilya R.; Gursky, Konstantin D.; Shishkov, Ivan N.; Narzaeva, Diana E.; Salmina, Alla B.; Yurchenko, Stanislav O.; Kryuchkov, Nikita P.
- Abstract
Astrocytes are glycolytically active cells in the central nervous system playing a crucial role in various brain processes from homeostasis to neurotransmission. Astrocytes possess a complex branched morphology, frequently examined by fluorescent microscopy. However, staining and fixation may impact the properties of astrocytes, thereby affecting the accuracy of the experimental data of astrocytes dynamics and morphology. On the other hand, phase contrast microscopy can be used to study astrocytes morphology without affecting them, but the post-processing of the resulting low-contrast images is challenging. The main result of this work is a novel approach for recognition and morphological analysis of unstained astrocytes based on machine-learning recognition of microscopic images. We conducted a series of experiments involving the cultivation of isolated astrocytes from the rat brain cortex followed by microscopy. Using the proposed approach, we tracked the temporal evolution of the average total length of branches, branching, and area per astrocyte in our experiments. We believe that the proposed approach and the obtained experimental data will be of interest and benefit to the scientific communities in cell biology, biophysics, and machine learning.
- Subjects
PHASE-contrast microscopy; ASTROCYTES; MACHINE learning; SCIENTIFIC community; CYTOLOGY; CENTRAL nervous system
- Publication
Scientific Reports, 2024, Vol 14, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-59773-2