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- Title
Continuous-release or burst-release of the antimicrobial peptide human lactoferrin 1-11 (hLF1-11) from calcium phosphate bone substitutes.
- Authors
Hein P. Stallmann; Christopher Faber; Eveline T. Slotema; D. M. Lyaruu; Antonius L. J. J. Bronckers; Arie V. Nieuw Amerongen; Paul I. J. M. Wuisman
- Abstract
Objectives: In order to identify possible drug delivery systems against resistant bone infection, we determined the release of the antimicrobial peptide (AMP) human lactoferrin 1-11 (hLF1-11) from commercially available bone substitutes. Methods: We combined six calcium phosphate cements and six granule-types with 5 mg/g hLF1-11 and measured its availability and release in vitro from cements (7 days) and granules (3 days). The integrity and antimicrobial activity of the hLF1-11 that was released during the first 24 h were measured, using mass spectrometry, and a killing assay on methicillin-resistant Staphylococcus aureus (MRSA). Results: Most of the cements showed burst release followed by low-level continuous release, whereas the coated granules showed high burst release for 24 h. After release the peptide was active (in nine of 12 materials) and intact. Conclusions: Different release profiles may be obtained by choosing the appropriate carrier, which supports the feasibility of biodegradable carriers releasing AMPs against resistant infections.
- Subjects
DRUG delivery devices; LACTOFERRIN; MASS spectrometry; STAPHYLOCOCCUS aureus; METHICILLIN
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2003, Vol 52, Issue 5, p853
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkg443