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- Title
Efficient mRNA Delivery with Lyophilized Human Serum Albumin-Based Nanobubbles.
- Authors
Kida, Hiroshi; Yamasaki, Yutaro; Feril Jr., Loreto B.; Endo, Hitomi; Itaka, Keiji; Tachibana, Katsuro
- Abstract
In this study, we developed an efficient mRNA delivery vehicle by optimizing a lyophilization method for preserving human serum albumin-based nanobubbles (HSA-NBs), bypassing the need for artificial stabilizers. The morphology of the lyophilized material was verified using scanning electron microscopy, and the concentration, size, and mass of regenerated HSA-NBs were verified using flow cytometry, nanoparticle tracking analysis, and resonance mass measurements, and compared to those before lyophilization. The study also evaluated the response of HSA-NBs to 1 MHz ultrasound irradiation and their ultrasound (US) contrast effect. The functionality of the regenerated HSA-NBs was confirmed by an increased expression of intracellularly transferred Gluc mRNA, with increasing intensity of US irradiation. The results indicated that HSA-NBs retained their structural and functional integrity markedly, post-lyophilization. These findings support the potential of lyophilized HSA-NBs, as efficient imaging, and drug delivery systems for various medical applications.
- Subjects
DRUG delivery systems; MESSENGER RNA; CONTRAST effect; SCANNING electron microscopy; GENE expression
- Publication
Nanomaterials (2079-4991), 2023, Vol 13, Issue 7, p1283
- ISSN
2079-4991
- Publication type
Article
- DOI
10.3390/nano13071283