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- Title
Application of UPLC-QTOF-MS Based Untargeted Metabolomics in Identification of Metabolites Induced in Pathogen-Infected Rice.
- Authors
Oh, Mira; Park, SeonJu; Kim, Hun; Choi, Gyung Ja; Kim, Seung Hyun
- Abstract
Metabolomics is a useful tool for comparing metabolite changes in plants. Because of its high sensitivity, metabolomics combined with high-resolution mass spectrometry (HR-MS) is the most widely accepted metabolomics tools. In this study, we compared the metabolites of pathogen-infected rice (Oryza sativa) with control rice using an untargeted metabolomics approach. We profiled the mass features of two rice groups using a liquid chromatography quadrupole time-of-flight mass spectrometry (QTOF-MS) system. Twelve of the most differentially induced metabolites in infected rice were selected through multivariate data analysis and identified through a mass spectral database search. The role of these compounds in metabolic pathways was finally investigated using pathway analysis. Our study showed that the most frequently induced secondary metabolites are prostanoids, a subclass of eicosanoids, which are associated with plant defense metabolism against pathogen infection. Herein, we propose a new untargeted metabolomics approach for understanding plant defense system at the metabolic level.
- Subjects
TIME-of-flight mass spectrometry; METABOLOMICS; RICE; PLANT metabolism; PLANT defenses
- Publication
Plants (2223-7747), 2021, Vol 10, Issue 2, p213
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants10020213