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- Title
流体剪切力对内皮细胞miR-21和miR-199a表达的影响.
- Authors
张燕; 王玉彩; 刘振东; 赵颖馨; 张华
- Abstract
To evaluate the effect of fluid shear stress on expression of microRNAs in endothelial cells.Low (4 dyn/cm2), middle (10 dyn/cm2), and high (15 dyn/cm2) fluid shear stress were loaded onto endothelial cells for 24 h using rotating cone disc shear stress system, respectively. No shear stress was loaded onto endothelial cells in control group. Changes of microRNAs expression were assessed using high throughput screening chip. The results were verified using quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatics analysis was performed in difference-expressed microRNAs.Compared to control group, there were 33 differentially expressed microRNAs in low shear stress group. Among them, 28 microRNAs expression were up-regulated and 5 microRNAs expression were down-regulated. In middle shear stress group, there were 8 differentially expressed microRNAs compared to control group. Among them, 6 microRNAs expression were up-regulated and 2 microRNAs expression were down-regulated. In high shear stress group, there were 31 microRNAs expression changed compared to control group. Among them, 25 microRNAs expression were up-regulated and 6 microRNAs expression were down-regulated.MiR-21 was markedly up-regulated in high shear stress group (fold change: 0.026) and significantly down-regulated in low shear stress group (fold change:3.531). MiR-199a was markedly up-regulated in low shear stress group (fold change: 0.075) and significantly down-regulated in high shear stress group (fold change:3.031). The results of bioinformatics analysis showed that target genes of differentially expressed microRNAs related to mechanical signal transduction, cell trans membrane transport, calcium ion signaling pathway, and endocytosis of cells.The change of the expressions of miR-21 and miR-199a were induced by fluid shear stress in endothelial cells.
- Publication
Chinese Journal of Bioinformatics, 2016, Vol 14, Issue 1, p19
- ISSN
1672-5565
- Publication type
Article
- DOI
10.3969/j.issn.1672-5565.2016.01.04