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- Title
Intranasal CpG-Oligodeoxynucleotide is a Potent Adjuvant of Vaccine againstHelicobacter pylori, and T Helper 1 Type Response and Interferon-γ Correlate with the Protection.
- Authors
Shi, Tong; Liu, Wen-Zhong; Gao, Fei; Shi, Gui-Ying; Xiao, Shu-Dong
- Abstract
Although a series of vaccines againstHelicobacter pylorihave emerged in the past 10 years, the mechanism involved in their protective effect is yet to be elucidated, and more effective vaccine adjuvants remain to be developed. In this study, CpG-oligodeoxynucleotide (CpG-ODN) was investigated as a new candidate for aH. pylorivaccine adjuvant. Furthermore, the role of T helper 1 (Th1) type response and interferon (IFN)-γ in the protective immunity was explored.C57BL/6 mice and IFN-γ knockout mice were intranasally or orally immunized withH. pyloriwhole cell sonicate (WCS)/CpG-ODN and challenged with different doses[5 × 108 and 5 × 106 colony-forming units (CFU)] ofH. pylori. The protective effect was assessed as the percentage of noninfected mice. The responsive antibodies and cytokines were analyzed using an enzyme-linked immunosorbent assay (ELISA) and flow cytometry.The prevention rates againstH. pyloriinfection in mice intranasally immunized with WCS plus CpG-ODN were dramatically higher than those in sham-immunized mice (70% vs. 0%, challenged with 5 × 108 CFUH. pylori; 90% vs. 20%, challenged with 5 × 106 CFUH. pylori). Significantly higher levels of immunoglobulin G2a (IgG2a) and IFN-γ were detected in the mice immunized with WCS/CpG than in sham-immunized controls. However, vaccination failed to effectively protect IFN-γ knockout mice challenged withH. pylori.CpG-ODN given intranasally is a potent adjuvant for development of aH. pylorivaccine. Th1-type response and IFN-γ are involved in the protection.
- Subjects
HELICOBACTER pylori infections; IMMUNOLOGICAL adjuvants; VACCINES; INTERFERONS; MICE; IMMUNITY
- Publication
Helicobacter, 2005, Vol 10, Issue 1, p71
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/j.1523-5378.2005.00293.x