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- Title
The effect of rebamipide on Helicobacter pylori extract-mediated changes of gene expression in gastric epithelial cells.
- Authors
Yoshida, N.; Ishikawa, T.; Ichiishi, E.; Yoshida, Y.; Hanashiro, K.; Kuchide, M.; Uchiyama, K.; Kokura, S.; Ichikawa, H.; Naito, Y.; Yamamura, Y.; Okanoue, T.; Yoshikawa, T.
- Abstract
Summary Background : Recent studies have shown that Helicobacter pylori affects intracellular signal transduction in host cells, leading to the activation of transcriptional factors and the induction of pro-inflammatory cytokines. On the other hand, rebamipide, an anti-gastritis and anti-ulcer agent, could scavenge reactive oxygen species and reduce interleukin-8 (IL-8) expression in gastric epithelial cells induced by H. pylori -stimulation through the attenuated activation of nuclear factor-κB (NF-κB). Aims : In this study, we investigated the effects of rebamipide on gene expression in H. pylori -stimulated epithelial cells using DNA chip. Methods : H. pylori water extract (HPE) was prepared from NCTC11637, the type strain of H. pylori . Total RNA was extracted from MKN45 cells, a human gastric cancer cell line, following HPE-stimulation with and without rebamipide for 3 h, and differences in gene expression profiles were observed using GeneChip and Human 6800 probe array. Results : The GeneChip analysis demonstrated that 132 up-regulated genes and 873 down-regulated genes, such as growth factors, chemokines and transcription factors, were detected in MKN45 cells 3 h after stimulation of H. pylori . Among them, several genes, including bFGF, RANTES and MIP-2β, were previously unknown to be expressed in H. pylori -stimulated human gastric cells. Rebamipide reduced expression of 119 genes encoding cytokines, growth factors and their receptors and transcription factors. Conclusions : These findings suggest that rebamipide could inhibit inflammatory reactions and tumour progression by modifying H. pylori infection-induced gene expression in gastric epithelial cells.
- Subjects
HELICOBACTER pylori; CELLULAR signal transduction; CYTOKINES
- Publication
Alimentary Pharmacology & Therapeutics, 2003, Vol 18, p63
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1046/j.1365-2036.18.s1.7.x