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- Title
Dynamics of photon-induced degradation and fluorescence in riboflavin microparticles.
- Authors
Pan, Y.-L.; Pinnick, R.G.; Hill, S.C.; Niles, S.; Holler, S.; Bottiger, J.R.; Wolf, J.-P.; Chang, R.K.
- Abstract
Abstract. An unexpected blue-fluorescence band (around 420nm) from both micrometer-sized dried particles and aqueous droplets of riboflavin [7,8-dimethyl-10-(D-1'-ribityl0-isoalloxazine] is observed when the microparticles are irradiated witha a pulsed UV (355- or 351-nm) laser. The intensity of the band increases quadratically with input laser energy density (fluence) and is attributable to a one-photon-exited fluorescence of lumichrome (7,8-dimethyl-alloxazine) that is produced by photdegradation of riboflavin increases sublinearly with UV-laser fluence. The well-known greenish-yellow fluorescence decays earlier and lumichrome fluorescence reaches a maximum later than the peak of the input laser pulse. The temporal dynamics of the 420- and 535-nm fluorescence peaks are consistent with a rate-equation simulation of photon-induced conversion of riboflavin to lumichrome and the subsequent fluorescence of lumichrome.
- Subjects
VITAMIN B2; FLAVINS; CHEMICAL reactions; LASERS
- Publication
Applied Physics B: Lasers & Optics, 2001, Vol 72, Issue 4, p449
- ISSN
0946-2171
- Publication type
Article
- DOI
10.1007/s003400100532