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- Title
Bob1 limits cellular frequency of T-follicular helper cells.
- Authors
Yamashita, Keiji; Kawata, Koji; Matsumiya, Hiroshi; Kamekura, Ryuta; Jitsukawa, Sumito; Nagaya, Tomonori; Ogasawara, Noriko; Takano, Ken‐ichi; Kubo, Terufumi; Kimura, Sachiko; Shigehara, Katsunori; Himi, Tetsuo; Ichimiya, Shingo
- Abstract
T follicular helper (Tfh) cells are involved in specific humoral immunity at initial and recall phases. The fact that the transcription repressors B-cell lymphoma-6 and Blimp-1 determine lineages of Tfh cells and other types of effector CD4+ T cells, respectively, suggests that there are unique mechanisms to establish Tfh-cell identity. In this study, we found that Tfh cells preferentially express the transcriptional coactivator Bob1. Bob1 of Tfh cells was dispensable for the expression of B-cell lymphoma-6 and the functional property of the cells for B cell help. However, upon initial immunization of foreign antigens, the percentages of Tfh cells in Bob1−/− mice were much higher than those in wild-type (WT) mice. In addition, expansion of Tfh cells within Bob1−/−CD4+ T cells transferred into WT mice revealed that the high frequency of Tfh cells was caused by a T-cell-intrinsic mechanism. These findings were further supported by the results of in vitro studies demonstrating that Bob1−/− Tfh cells had greater proliferative activity in response to stimuli by CD3/CD28 monoclonal antibody and were also refractory to CD3-induced cell death in comparison to WT Tfh cells. These results suggest that Tfh cells harbor a Bob1-related mechanism to restrict numerical frequency against stimulation of TCRs.
- Publication
European Journal of Immunology, 2016, Vol 46, Issue 6, p1361
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.201545499