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- Title
B-1 cells in the bone marrow are a significant source of natural IgM.
- Authors
Choi, Youn Soo; Dieter, Jacquelyn A.; Rothaeusler, Kristina; Luo, Zheng; Baumgarth, Nicole
- Abstract
Natural IgM antibodies secreted in the absence of antigenic challenge are important contributors to antimicrobial immunity and tissue homeostasis. Early studies identified BM and, to a lesser extent the spleen, as main tissue sources of this spontaneously secreted IgM. However, the responsible B-cell subset has never been identified. Using multicolor flow cytometry, cell sorting and chimeric mice in which B-1 and B-2 cells and their secreted antibodies are distinguished by their Ig-allotype, we unequivocally identify the natural IgM-secreting cells in spleen and, for the first time, in the BM as IgM+ IgDlo/-CD19hi CD43+ CD5+/− B-1 cells. The newly identified population of BM B-1 cells shows many of the phenotypic characteristics of splenic B-1 cells but is distinct from B-1 cells in the peritoneal cavity, which generate at best very small amounts of IgM. Antibody-secreting spleen and BM B-1 cells are distinct also from terminally differentiated plasma cells generated from antigen-induced conventional B cells, as they express high levels of surface IgM and CD19 and lack expression of CD138. Overall, these data identify populations of non-terminally differentiated B-1 cells in spleen and BM as the most significant producers of natural IgM.
- Publication
European Journal of Immunology, 2012, Vol 42, Issue 1, p120
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.201141890