We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
IL-7 is essential for lymphopenia-driven turnover of colitogenic CD4.
- Authors
Tomita, Takayuki; Kanai, Takanori; Totsuka, Teruji; Nemoto, Yasuhiro; Okamoto, Ryuichi; Tsuchiya, Kiichiro; Sakamoto, Naoya; Ohteki, Toshiaki; Hibi, Toshifumi; Watanabe, Mamoru
- Abstract
We previously demonstrated that IL-7 is essential for the persistence of T-cell-mediated colitis, by showing that adoptive transfer of CD4CD45RB T cells into IL-7×RAG-1 mice did not induce colitis; and that intestinal IL-7 is not essential for this colitis model, by showing that IL-7×RAG-1 mice parabiosed with colitic CD4CD45RB T-cell-transferred RAG-1 mice developed colitis. Here, we investigated the role of IL-7 in the maintenance of colitogenic CD4 T cells by surgically separating these parabionts. Surprisingly, the separated IL-7×RAG-1 mice were consistently diseased after separation, although no IL-7 mRNA was detected in the tissues of separated IL-7×RAG-1 partners. CD4 T cells isolated from the separated RAG-1 or IL-7×RAG-1 mice were then transferred into new RAG-1 or IL-7×RAG-1 mice. Regardless of the source of donor cells, RAG-1 recipients developed colitis, whereas IL-7×RAG-1 recipients did not. Collectively, these results demonstrate that IL-7 is essential for lymphopenia-driven turnover of colitogenic CD4 T cells rather than the maintenance of those cells in established colitic mice. They also provide a basis for the timing of IL-7/IL-7R blockade for the treatment of inflammatory bowel diseases.
- Publication
European Journal of Immunology, 2009, Vol 39, Issue 10, p2737
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200838905