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- Title
Increased detection of proliferating, polyfunctional, HIV-1-specific T cells in DNA-modified vaccinia virus Ankara-vaccinated human volunteers by cultured IFN-γ ELISPOT assay.
- Authors
Winstone, Nicola; Guimarães-Walker, Ana; Roberts, Joanna; Brown, Denise; Loach, Vanessa; Goonetilleke, Nilu; Hanke, Tomáš; McMichael, Andrew J.
- Abstract
Induction of a long-term immunological memory, which can expand and defend the host upon pathogen encounter, is the 'holy grail' of vaccinology. Here, using a sensitive cultured IFN-γ ELISPOT assay, we show that 50% (15 out of 30) of healthy, HIV-1/2-uninfected volunteers who received pTHr.HIVA DNA prime-modified vaccinia virus Ankara. HIVA boost vaccine regimen 1 to 3 1/2 years ago had detectable HIV-1-specific T-cell responses. These T cells, predominantly of the CD4 subtype, could proliferate and produce multiple cytokines in response to in vitro peptide stimulation. Peptide mapping studies showed that the vaccine-induced CD4 T cells were mostly directed toward epitopes targeted in HIV-1-infected individuals. In addition, we used the same assay to re-evaluate 51 volunteers from past vaccine trial IAVI-006 and corrected the previously reported 10% of vaccine responders to 50%. Thus, we confirmed that cultured assays are a valuable tool for studying T-cell memory. These results are discussed in the context of the current state-of-affairs of the HIV-1 vaccine field.
- Publication
European Journal of Immunology, 2009, Vol 39, Issue 4, p975
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200839167