We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Broad analysis reveals a consistent pattern ofvargene transcription inPlasmodium falciparumrepeatedly selected for a defined adhesion phenotype.
- Authors
Duffy, Michael F.; Byrne, Timothy J.; Elliott, Salenna R.; Wilson, Danny W.; Rogerson, Stephen J.; Beeson, James G.; Noviyanti, Rintis; Brown, Graham V.
- Abstract
Transcription of the majority of the members of thePlasmodium falciparum varmultigene family were analysed in two isolates by a quantitative approach. Both of these isolates had been repeatedly selected for adhesion to chondroitin sulphate A (CSA) and one had also been selected for adhesion to hyaluronic acid (HA). These adhesion phenotypes are expressed by many parasites isolated from placentae and are associated with malaria disease in pregnancy. Increased transcription of thevargenevar2csa, or its homologue IT4 var4, was associated with the CSA and HA adhesion phenotypes in all parasites suggesting that it was the dominant, if not the only,vargene that encoded adhesion to CSA in these allogeneic isolates. Somevargenes were consistently transcribed at higher levels than others regardless of expressed adhesion phenotypes suggesting a transcriptional hierarchy. Unspliced or partial transcripts were detected for mostvargenes tested. These atypicalvargene transcripts may have implications for the regulation ofvargene transcription.
- Subjects
PLASMODIUM falciparum; GENOTYPE-environment interaction; ADHESION; PARASITES; GENETICS; MALARIA
- Publication
Molecular Microbiology, 2005, Vol 56, Issue 3, p774
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.2005.04577.x