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- Title
The synthesis and application of a colour-switch β-arylethenesulfonyl fluoride fluorescent probe in the detection of serum albumin.
- Authors
Giel, Marie-Claire; Owyong, Tze Cin; Hong, Yuning
- Abstract
Proteins play a pivotal role in regulating important physiological processes and serve as important biomarkers for many diseases. Herein, we present a new strategy for bovine serum albumin (BSA) detection using a novel colour-switch fluorescent probe CPV-ESF ((E)-2-(4-((Z)-1-cyano-2-(4-(diethylamino)phenyl)vinyl)phenyl)ethene-1-sulfonyl fluoride). CPV-ESF reacts with nucleophilic amino acids of BSA via 1,4-Michael addition click chemistry to create a covalently linked CPV-ESF:BSA complex, which can be easily detected by a fluorescence colour-switch response. The sensing mechanism, sensitivity and selectivity of CPV-ESF for BSA detection as well as its application for cell imaging have been investigated. This work reports the development of a small-molecule fluorescent probe bearing the unique β-arylethenesulfonyl fluoride unit for fluorescence colour switching and its application for the labelling and detection of bovine serum albumin.
- Subjects
SERUM albumin; FLUORESCENT probes; CLICK chemistry; CELL imaging; AMINO acids
- Publication
Australian Journal of Chemistry, 2022, Vol 75, Issue 11, p877
- ISSN
0004-9425
- Publication type
Article
- DOI
10.1071/CH22165