We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
RepA negatively autoregulates the transcription of the repABC operon of the Rhizobium etli symbiotic plasmid basic replicon.
- Authors
Ramírez-Romero, Miguel A.; Téllez-Sosa, Juan; Barrios, Humberto; Pérez-Oseguera, Angeles; Rosas, Vania; Cevallos, Miguel A.
- Abstract
The basic replicon of Rhizobium etli CE3, like other members of the repABC plasmid family, is constituted by the repABC operon. RepC is essential for replication, and RepA and RepB play a role in plasmid segregation. It has been shown that deletion derivatives lacking the repAB genes have an increased copy number, indicating that these genes participate in the control of plasmid copy number. RepA is also a trans-incompatibility factor. To understand the regulation of the repABC operon, in this paper: (i) the transcription start site of the repABC operon was determined; (ii) the promoter region was identified by site-directed mutagenesis of the putative -35 and -10 hexameric elements; and (iii) RepA was recognized as a negative regulator of the transcription of the repABC operon.
- Subjects
RHIZOBIUM; GENETIC transcription; MOBILE genetic elements; GENETIC mutation; RADIOGENETICS; MICROORGANISMS
- Publication
Molecular Microbiology, 2001, Vol 42, Issue 1, p195
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1046/j.1365-2958.2001.02621.x