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- Title
Regulation of nitrogen metabolism is altered in a glnB mutant strain of Rhizobium leguminosarum.
- Authors
Amar, M.; Patriarca, E. J.; Manco, G.; Bernard, P.; Riccio, A.; Lamberti, A.; Defez, R.; Iaccarino, M.
- Abstract
We isolated a <em>Rhizobium leguminosarum</em> mutant strain altered in the <em>glnB</em> gene. This event, which has never been described in the Rhizobieceae, is rare in comparison to mutants Isolated In the contiguous gene, <em>glnA</em>. The <em>glnB</em> mutation removes the <em>glnBA</em> promoter but <em>in vivo</em> does not prevent <em>glnA</em> expression from its own promoter, which is not nitrogen regulated. The <em>glnB</em> mutant strain does not grow on nitrate as a sole nitrogen source and it is Nod+, Fix+. Two -24/-12 promoters, for the <em>glnll</em> and <em>glnBA</em> genes, are constitutively expressed in the <em>glnB</em> mutant, while two -35/-10-like promoters for <em>glnA</em> and <em>ntrBC</em> are unaffected. We propose that the <em>glnB</em> gene product, the Pll protein, plays a negative role in the ability of NtrC to activate transcription from its target promoters and a positive role in the mechanism of nitrate utilization.
- Subjects
RHIZOBIUM leguminosarum; GENES; MICROBIAL mutation; GENE expression; NITROGEN
- Publication
Molecular Microbiology, 1994, Vol 11, Issue 4, p685
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1994.tb00346.x