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- Title
Aflatoxin B<sub>1</sub> Negatively Regulates Wnt/β-Catenin Signaling Pathway through Activating miR-33a.
- Authors
Fang, Yi; Feng, Youjun; Wu, Tongjin; Srinivas, Swaminath; Yang, Weiqiang; Fan, Jue; Yang, Chi; Wang, Shihua
- Abstract
MicroRNAs are known to play an important role in modulating gene expression in various diseases including cancers and cardiovascular disorders, but only a few of them are associated with the pathology of aflatoxin B1 (AFB1), a potent mycotoxin. Here, we discovered a novel regulatory network between AFB1, miR-33a and β-catenin in human carcinoma cells. The level of miR-33a was up-regulated in hepatocellular carcinoma (HCC) cells treated with AFB1, while in the same cells causing the decrease in β-catenin expression when treated at their IC50 values. miR-33a, specifically miR-33a-5p, was demonstrated to down-regulate the expression of β-catenin, affect the β-catenin pathway, and inhibit cell growth. Also, by employing a luciferase assay, we found that miR-33a down-regulated β-catenin by directly binding to the 3’-UTR of β-catenin. These results suggested that AFB1 might down-regulate β-catenin by up-regulating miR-33a. This understanding opens new lines of thought in the potential role of miR-33a in the clinical therapy of cancer.
- Subjects
AFLATOXINS; WNT genes; CATENINS; GENE expression; MICRORNA; CELLULAR signal transduction; MYCOTOXINS
- Publication
PLoS ONE, 2013, Vol 8, Issue 8, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0073004