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- Title
Expression of High-Affinity IgE Receptor on Human Peripheral Blood Dendritic Cells in Children.
- Authors
Vasudev, Monica; Cheung, Dorothy S.; Pincsak, Hannah; Shun-Hwa Li; Ke Yan; Simpson, Pippa; Dasu, Trivikram; Grayson, Mitchell H.
- Abstract
Background: In a mouse model of viral induced atopic disease, expression of FcϵRI on dendritic cells is critical. While adult human conventional (cDC) and plasmacytoid (pDC) dendritic cells have been shown to express FcϵRI, it is not known if this receptor is expressed in childhood and how its expression is governed by IgE. Methods: Following informed consent of subjects (n = 27, aged 12-188 months), peripheral blood was stained for surface expression of CD19, ILT7, CD1c, IgE, FcϵRI and analyzed by flow cytometry (cDC: CD19- ILT7- CD1c+; pDC: CD19- ILT7+ CD1c-). Total and specific serum IgE levels to food and inhalant allergens were determined by ImmunoCAP, and the relationship between FcϵRI expression on dendritic cells and sensitization, free IgE, cell bound IgE, and age was determined. Results: Independent of sensitization status, FcϵRI expression was noted on cDC and pDC as early as 12 months of age. Serum IgE level correlated with expression of FcϵRI on cDC, but not pDC. Based on the concentration of IgE, a complex relationship was found between surface bound IgE and expression of FcϵRI on cDC. pDC exhibited a linear relationship of FceRI expression and bound IgE that was consistent through all IgE concentrations. Conclusions: In children, FcϵRI expression on cDC and pDC is modulated differently by serum and cell bound IgE. IgE governance of FcϵRI expression on cDC depends upon a complex relationship. Further studies are needed to determine the functional roles of FceRI on cDC and pDC.
- Subjects
IMMUNOGLOBULIN E receptors; DENDRITIC cells; ANTIGEN presenting cells; GENE expression; ATOPIC dermatitis; JUVENILE diseases; PLASMACYTOMA; LABORATORY mice
- Publication
PLoS ONE, 2012, Vol 7, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0032556