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- Title
A direct protein kinase B-targeted anti-inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris.
- Authors
Ju Young Yoon; Ji Hye Kim; Kwang-Soo Baek; Geum Soog Kim; Seung Eun Lee; Dae Young Lee; Je Hun Choi; Seung Yu Kim; Hyun Bong Park; Gi-Ho Sung; Kang Ro Lee; Jae Youl Cho; Hyung Jun Noh
- Abstract
Background: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities. Objective: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities. Materials and Methods: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays. Results: From Cm-EE, 13 constituents, including trehalose (1), cordycepin (2), 6-hydroxyethyladenosine (3), nicotinic amide (4), butyric acid (5), β-dimorphecolic acid (6), a-dimorphecolic acid (7), palmitic acid (8), linoleic acid (9), cordycepeptide A (10), 4-(2-hydroxy-3-((9E,12E)-octadeca-9,12-dienoyloxy)propoxy)-2- (trimethylammonio)butanoate (11), 4-(2-hydroxy-3-(palmitoyloxy)propoxy)-2-(trimethylammonio) butanoate (12), and linoleic acid methyl ester (13) were isolated. Of these components, compound 2 displayed a significant inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW264.7 cells. Furthermore, this compound strongly and directly suppressed the kinase activity of AKT, an essential signalling enzyme in LPS-induced NO production, by interacting with its ATP binding site. Conclusion: C. militaris could have anti-inflammatory activity mediated by cordycepin-induced suppression of AKT.
- Subjects
PROTEIN kinase B; CORDYCEPS; COMPOSITION of mushrooms; ANTI-inflammatory agents; PLANT extracts; ETHANOL
- Publication
Pharmacognosy Magazine, 2015, Vol 11, Issue 43, p477
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/0973-1296.160454