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- Title
Multiple DNA Markers for Evaluation of Resistance against Potato virus Y, Potato virus S and Potato leafroll virus.
- Authors
NADERPOUR, MASOUD; SADEGHI, LEILA
- Abstract
Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCRbased markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.
- Subjects
PLANT DNA; POTYVIRIDAE; GENETIC markers in plants; POTATO disease &; pest resistance; PLANT breeding; POTATOES
- Publication
Czech Journal of Genetics & Plant Breeding, 2018, Vol 54, Issue 1, p30
- ISSN
1212-1975
- Publication type
Article
- DOI
10.17221/180/2016-CJGPB