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- Title
Optimization of extraction process and antioxidant activities of saponins from Camelliafascicularis leaves.
- Authors
Hu, Xiang; Tang, Jun-rong; Zhang, Gui-liang; Deng, Jia; Kan, Huan; Zhang, Ying-jun; Zhao, Ping; Liu, Yun
- Abstract
Saponins from Camelliafascicularis leaves, which ultrasonic-assisted extraction process parameters were optimized with Box–Behnken design and antioxidant activities were assessed, were reported in this study. The highest yield of crude saponins was up to 3.68% when the optimal extraction conditions were ratio of solvent to solid of 52.1 mL/g, methanol concentration of 71.1%, and ultrasonic temperature of 50.3 °C. The crude saponins from C.fascicularis leaves were purified using HP-20 macroporous resin with methanol and three fractions (SCF-1, SCF-2 and SCF-3) were obtained. Subsequently, the in vitro scavenging activity assay showed that the fractions proved significant antioxidant capacities as dose-dependent manner on 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). SCF-1 possessed significantly higher antioxidant activity than that SCF-2 and SCF-3. Overall, ultrasonic-assisted extraction could be effectively used to extract C.fascicularis leaves saponins, which possessed potential antioxidant capabilities and is expected to be developed as food materials.
- Subjects
SAPONINS; EXTRACTION (Chemistry); CHEMICAL yield; METHANOL; SOLVENTS
- Publication
Journal of Food Measurement & Characterization, 2021, Vol 15, Issue 2, p1889
- ISSN
2193-4126
- Publication type
Article
- DOI
10.1007/s11694-020-00754-0