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- Title
Intracoronary Infusion of CD133<sup>+</sup> and CD133<sup>-</sup>CD34<sup>+</sup> Selected Autologous Bone Marrow Progenitor Cells in Patients with Chronic Ischemic Cardiomyopathy: Cell Isolation, Adherence to the Infarcted Area, and Body Distribution.
- Authors
Goussetis, Evgenios; Manginas, Athanassios; Koutelou, Maria; Peristeri, Ioulia; Theodosaki, Maria; Kollaros, Nikolaos; Leontiadis, Evangelos; Theodorakos, Athanasios; Paterakis, George; Cokkinos, Dennis V.; Graphakos, Stelios
- Abstract
Central issues in intracoronary infusion (ICI) of bone marrow (BM)-cells to damaged myocardium for improving cardiac function are the cell number that is feasible and safe to be administrated as well as the retention of cells in the target area. Our study addressed these issues in eight patients with chronic ischemic cardiomyopathy undergoing ICI of selected BM-progenitors. We could immunomagnetically isolate 0.8 ± 0.32 × 107 CD133+ cells and 0.75 ± 0.24 × 107 CD133-CD34+ cells from 310 ± 40 ml BM. After labeling these cells with 99mTc-hexamethylpropylenamineoxime, they were infused into the infarct-related artery without any complication. Scintigraphic images 1 (eight patients) and 24 hours (four patients) after ICI revealed an uptake of 9.2% ± 3.6 and 6.8% ± 2.4 of the total infused radioactivity in the infarcted area of the heart, respectively; the remaining activity was distributed mainly to liver and spleen. We conclude that through ICI of CD133+ and CD133-CD34+ BM-progenitors a significant number of them are preferentially attracted to and retained in the chronic ischemic myocardium.
- Subjects
CARDIOMYOPATHIES; BONE marrow; ISCHEMIA; BLOOD circulation disorders; BLOOD vessels; BILIARY tract; LYMPHOID tissue; STEM cell research
- Publication
Stem Cells, 2006, Vol 24, Issue 10, p2279
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2005-0589