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- Title
IMMUNOPHENOTYPIC PROFILE OF ACUTE MYELOID LEUKEMIA BY FLOW CYTOMETRY.
- Authors
M. Y., Shafini; B., Rosnah; M. N., Noor Haslina; W. M., Wan Haslindawani
- Abstract
INTRODUCTION : Acute myeloid leukemia (AML) is a group of neoplastic disorders characterized by the proliferation and accumulation of immature hematopoietic cells in the bone marrow and blood. AML accounts for approximately 20% of acute leukemia in children and 80% of acute leukemia in adults. Over the last decade, immunophenotyping has become extremely important not only in determining the lineage of the acute leukemias but also in the detection of mixed lineage leukemias and minimal residual disease. It is also suggested as having a prognostic significance. Our objective was to study the immunophenotypic profile of acute myeloid leukemia at diagnosis. METHODOLOGY : This is a retrospective analysis of 53 cases of AML diagnosed at our institution between 2004 and 2005. Diagnosis was based on peripheral blood and bone marrow examination for morphology, cytochemistry and immunophenotypic studies. Immunophenotyping was performed by tricolour flow cytometry analysis using CD45/SSC gating. SPSS version 10 software package was used for statistical analysis. RESULTS : AML accounted for 35.2% of all acute leukemias and 87% were adult AML. The commonest FAB subtype was AML-M2 (31.6%). CD13 and CD33 were most commonly present in all AML subtypes. CD14 was most often seen in monocytic and myelomonocytic (75%) leukemias. Lymphoid antigen expression was seen in 41% of cases. CD4 expression was the commonest (23%). CONCLUSION : AML accounted for over a third (35.2%) of all leukemias diagnosed at our institution between 2004 and 2005, and adults accounted for a large percentage (87%). The commonest FAB subtype was AML M2. CD13 and CD33 were the most useful markers in the diagnosis of AML. Aberrant lymphoid expression was seen in less than half (41%) of these cases and CD4 was the lymphoid marker most often expressed.
- Subjects
ACUTE myeloid leukemia; BONE marrow; LEUKEMIA; LYMPHOID tissue; ANTIGENS
- Publication
Malaysian Journal of Medical Sciences, 2006, Vol 13, p206
- ISSN
1394-195X
- Publication type
Article