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- Title
Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)-octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells.
- Authors
Kang, Woo-Ri; Seo, Min-Ju; An, Jung-Ung; Shin, Kyung-Chul; Oh, Deok-Kun
- Abstract
Objective: To produce δ-decalactone from linoleic acid by one-pot reaction using linoleate 13-hydratase with supplementation with whole Yarrowia lipolytica cells. Results: Whole Y. lipolytica cells at 25 g l produced1.9 g l δ-decalactone from 7.5 g 13-hydroxy-9(Z)-octadecenoic acid l at pH 7.5 and 30 °C for 21 h. Linoleate 13-hydratase from Lactobacillus acidophilus at 3.5 g l with supplementation with 25 g Y. lipolytica cells l in one pot at 3 h produced 1.9 g l δ-decalactone from 10 g linoleic acid l via 13-hydroxy-9(Z)-octadecenoic acid intermediate at pH 7.5 and 30°C after 18 h, with a molar conversion yield of 31 % and productivity of 106 mg l h. Conclusion: To the best of our knowledge, this is the first production of δ-decalactone using unsaturated fatty acid.
- Subjects
LINOLEIC acid; OLEIC acid; HYDRATASES; DIPODASCACEAE; UNSATURATED fatty acids
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 5, p817
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2041-3