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- Title
Characterization and expression of glucosamine-6-phosphate synthase from Saccharomyces cerevisiae in Pichia pastoris.
- Authors
Wang, Sheng; Li, Piwu; Su, Jing; Wu, Xiangkun; Liang, Rongrong
- Abstract
Glucosamine-6-phosphate (GlcN-6-P) synthase from Saccharomyces cerevisiae was expressed in Pichia pastoris SMD1168 GIVING maximum activity of 96 U ml for the enzyme in the culture medium. By SDS-PAGE, the enzyme, a glycosylated protein, had an apparent molecular mass of 90 kDa. The enzyme was purified by gel exclusion chromatography to near homogeneity, with a 90 % yield and its properties were characterized. Optimal activities were at pH 5.5 and 55 °C, respectively, at which the highest specific activity was 6.8 U mg protein . The enzyme was stable from pH 4.5 to 5.5 and from 45 to 60 °C. The K and V of the GlcN-6-P synthase towards d-fructose 6-phosphate were 2.8 mM and 6.9 μmol min mg, respectively.
- Subjects
GLUCOSAMINE; GENE expression; SACCHAROMYCES cerevisiae; PICHIA pastoris; GLYCOSYLATION
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 10, p2023
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1561-y