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- Title
Real-time monitoring of extracellular matrix-mediated PC12 cell attachment and proliferation using an electronic biosensing device.
- Authors
Zhang, Yan; Wang, Lei; Zhu, Jing; Hu, Yadong; Xing, Wanli; Cheng, Jing
- Abstract
The attachment and proliferation of a well-established, neuron-like cell line, rat pheochromocytoma (PC12) cells, on different extracellular matrices (ECMs) was monitored using cellular impedance sensing (CIS). Commonly used ECMs, including fibronectin, laminin, poly- l-lysine, collagen and poly- l-lysine followed by laminin, in addition to DMEM cell culture media alone as a control, were studied: CIS identified the dynamic progress of the adhesion and proliferation of the cells on different ECMs. Among these modified ECM surfaces, the laminin- and poly- l-lysine/laminin-modified surfaces were the best suited for the neuron-to-electrode surface attachment and proliferation, which was confirmed by MTT assays and a scanning electron microscopy analysis. This work provides a simple method to study neuron cell/ECM interactions in a real-time, label-free, and quantitative manner.
- Subjects
PHEOCHROMOCYTOMA; EXTRACELLULAR matrix; CONNECTIVE tissues; CELL culture; COLLAGEN; NEUROENDOCRINE tumors
- Publication
Biotechnology Letters, 2012, Vol 34, Issue 2, p397
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-011-0773-7