We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius.
- Authors
Thompson, Ann H.; Studholme, David J.; Green, Edward M.; Leak, David J.
- Abstract
Expression of a pyruvate decarboxylase (Pdc) pathway in metabolically versatile thermophilic bacteria could create novel ethanologenic organisms, but no suitable thermostable Pdc is available. We have demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillus thermoglucosidasius at up to 52°C, while expression of Pdc polypeptides up to 54°C was evident from Western blotting. By using an unstable lactate dehydrogenase ( ldh) mutant of G. thermoglucosidasius, indirect evidence of Pdc activity in vivo was also obtained.
- Subjects
PYRUVATES; THERMOPHILIC bacteria; ZYMOMONAS mobilis; POLYPEPTIDES; WESTERN immunoblotting; LACTATE dehydrogenase; GENETIC mutation; ANAEROBIC bacteria; KETONIC acids
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 8, p1359
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9698-1