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- Title
Enhanced tolerance of the rat myocardium to ischemia and reperfusion injury early after acute myocardial infarction.
- Authors
Pantos, Constantinos; Mourouzis, Iordanis; Dimopoulos, Antonios; Markakis, Konstantinos; Panagiotou, Matthew; Xinaris, Christodoulos; Tzeis, Stylianos; Kokkinos, Alexandros; Cokkinos, Dennis V.
- Abstract
It is now recognized that changes occurring during cardiac remodeling may influence the tolerance of the myocardium to ischemic stress. Therefore, the present study investigated the response of the post-infarcted heart to ischemia in an experimental model of ischemia and reperfusion injury and the possible underlying mechanisms. Acute myocardial infarction (AMI) was induced in Wistar male rats by ligating the left coronary artery (AMI, n = 13), while sham-operated rats were used as controls (SHAM, n = 11). At 2 weeks, cardiac dysfunction was observed in AMI, as indicated by the reduction of the left ventricular EF%. Isolated hearts were then subjected to 30 min of zero-flow global ischemia followed by 45 min of reperfusion. Ischemic contracture was significantly depressed in AMI hearts. Postischemic left ventricular end diastolic pressure (LVEDP45) in mmHg and LDH release in IU/g were markedly decreased; LVEDP45 was 52.1 (7.5) for AMI vs 96.6 (7.5),P < 0.05 and LDH release was 7.5 (1.0) in AMI vs 11.4 (0.56) in SHAM, P < 0.05. This response was associated with 2-fold increase in HSP70 expression in AMI hearts (noninfarcted segment), P < 0.05 vs SHAM and 1.7 fold increase in the expression of the phospho-HSP27, P < 0.05, while the expression of PKCε was shown to be 1.4-fold less in AMI, P < 0.05. In conclusion, the post-infarcted heart seems to be resistant to ischemiareperfusion injury and heat shock protein 70 and 27 may be involved in this response.
- Subjects
ISCHEMIA; MYOCARDIAL infarction; CORONARY arteries; HEAT shock proteins; BLOOD circulation disorders; CARDIOGENIC shock
- Publication
Basic Research in Cardiology, 2007, Vol 102, Issue 4, p327
- ISSN
0300-8428
- Publication type
Article
- DOI
10.1007/s00395-007-0645-4