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- Title
Development of ten microsatellite markers for Quercus mongolica var. crispula by database mining.
- Authors
Ueno, Saneyoshi; Tsumura, Yoshihiko
- Abstract
Simple sequence repeats (SSRs) in the NCBI dbEST database were surveyed to identify potential SSR markers for Quercus mongolica. In total, 2,691 gene sequences, mainly from expressed sequence tags (ESTs) for Q. robur and Q. petraea had been registered. Twenty-two PCR primers were designed for SSRs in these sequences and screened for polymorphisms in 16 Q. mongolica trees. Ten loci were easily genotyped and showed polymorphism, with numbers of alleles and expected heterozygosity ranging from 3 to 15 and 0.28 to 0.94, respectively. These EST-SSR markers should be useful for studying the genetic diversity of Quercus species.
- Subjects
MICROSATELLITE repeats; GENETIC engineering; MONGOLIAN oak; TISSUE culture; SPECIES diversity; GENETIC polymorphisms; MACHINE learning; NANOTECHNOLOGY; SCIENTIFIC experimentation
- Publication
Conservation Genetics, 2008, Vol 9, Issue 4, p1083
- ISSN
1566-0621
- Publication type
Article
- DOI
10.1007/s10592-007-9462-4