We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Crystallographic analysis of a subcomplex of the transsulfursome with tRNA for Cys-tRNA<sup>Cys</sup> synthesis.
- Authors
Meirong Chen; Yuto Nakazawa; Yume Kubo; Nozomi Asano; Koji Kato; Isao Tanaka; Min Yao
- Abstract
In most organisms, Cys-tRNACys is directly synthesized by cysteinyl-tRNA synthetase (CysRS). Many methanogenic archaea, however, use a two-step, indirect pathway to synthesize Cys-tRNACys owing to a lack of CysRS and cysteine-biosynthesis systems. This reaction is catalyzed by O-phosphoseryl-tRNA synthetase (SepRS), Sep-tRNA:Cys-tRNA synthase (SepCysS) and SepRS/SepCysS pathway enhancer (SepCysE) as the transsulfursome, in which SepCysE connects both SepRS and SepCysS. On the transsulfursome, SepRS first ligates an O-phosphoserine to tRNACys, and the mischarged intermediate Sep-tRNACys is then transferred to SepCysS, where it is further modified to Cyst-RNACys. In this study, a subcomplex of the transsulfursome with tRNACys (SepCysS-SepCysE-tRNACys), which is involved in the second reaction step of the indirect pathway, was constructed and then crystallized. The crystals diffracted X-rays to a resolution of 2.6 Å and belonged to space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1 Å . The structure determined by molecular replacement showed that the complex consists of a SepCysS dimer, a SepCysE dimer and one tRNACys in the asymmetric unit.
- Subjects
TRANSFER RNA; CYSTEINE
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2016, Vol 72, Issue 7, p569
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X16009559