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- Title
Membrane attack complex generation increases as a function of time in stored blood.
- Authors
Hu, X.; Patel, R. P.; Weinberg, J. A.; Marques, M. B.; Ramos, T. N.; Barnum, S. R.
- Abstract
SUMMARY Objective To determine if the complement system, a potent mediator of inflammation, contributes to haemolysis during red blood cell ( RBC) storage. Background RBCs in storage undergo structural and biochemical changes that may result in adverse patient outcomes post-transfusion. Complement activation on leukodepletion and during storage may contribute to the RBC storage lesion. Methods/Materials We performed a cross-sectional analysis of aliquots of leukoreduced RBC units, stored for 1-6 weeks, for the levels of C3a, C5a, Bb, iC3b, C4d and C5b-9 [membrane attack complex ( MAC)] by enzyme-linked immunosorbent assay ( ELISA). Results We observed that only MAC levels significantly increased in RBC units as a function of storage time. We also observed that the level of C5b-9 bound to RBCs increased as a function of storage time. Conclusion MAC levels increased over time, suggesting that MAC is the primary complement-mediated contributor to changes in stored RBCs. Inhibition of the terminal complement pathway may stabilise RBC functionality and extend shelf life.
- Subjects
ERYTHROCYTES; HEMOLYSIS &; hemolysins; ALIQUOTS (Chemistry); ENZYME-linked immunosorbent assay
- Publication
Transfusion Medicine, 2014, Vol 24, Issue 2, p114
- ISSN
0958-7578
- Publication type
Article
- DOI
10.1111/tme.12109