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- Title
Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient Kit<sup>W-sh/W-sh</sup> mice.
- Authors
Shohei Shibagaki; Satoko Tahara-Hanaoka; Takashi Hiroyama; Yukio Nakamura; Akira Shibuya
- Abstract
Mast cells (MCs) play pivotal roles in allergic reactions and the host defense against microbial infection through the IgE-dependent and IgE-independent signaling pathways. MC lines that can be analyzed both in vitro and in vivo would be useful for the study of MC-dependent immune responses. Here, we investigated the functional characteristics of a mouse embryonic stem cell-derived MC-like cell line, MEDMC-BRC6. The cell line expressed FcεRI and c-Kit and showed degranulation and production of inflammatory cytokines and chemokines, including TNF-α, IL-6 and MCP-1, upon cross-linking Fcε RI with IgE. These cytokines and chemokines were also produced by the cell line by stimulation of TLR2 and TLR4. MEDMC-BRC6 survived in the peritoneal cavity and the ear skin for at least 6 months after the transfer into genetically compatible MC-deficient KitW-sh/W-sh mice, in which systemic anaphylaxis was successfully induced. Thus, MEDMC-BRC6 cells represent a potent tool for investigating the functions of MCs in vitro and in vivo.
- Subjects
MAST cells; CHEMOKINES; CYTOKINES; IMMUNE response; LABORATORY mice
- Publication
International Immunology, 2017, Vol 29, Issue 5, p235
- ISSN
0953-8178
- Publication type
Article
- DOI
10.1093/intimm/dxx022