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- Title
Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells.
- Authors
Fraire, Juan C.; Shaabani, Elnaz; Sharifiaghdam, Maryam; Rombaut, Matthias; Hinnekens, Charlotte; Hua, Dawei; Ramon, Jana; Raes, Laurens; Bolea-Fernandez, Eduardo; Brans, Toon; Vanhaecke, Frank; Borghgraef, Peter; Huang, Chaobo; Sauvage, Félix; Vanhaecke, Tamara; De Kock, Joery; Xiong, Ranhua; De Smedt, Stefaan; Braeckmans, Kevin
- Abstract
Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles. Upon irradiation with pulsed laser light, fast heating of the core particle results in vapor bubble formation, which propels the nanoprojectiles through the cell membrane of nearby cells. We show successful transfection of both adherent and non-adherent cells with mRNA and pDNA, outperforming electroporation as the most used physical transfection technology by a factor of 5.5–7.6 in transfection yield. With a throughput of 104-105 cells per second, biolistic delivery with NBs offers scalable and highly efficient transfections of mammalian cells. Ballistic delivery with micro/nano-particles has been successfully used to transfect plant cells, however, has failed in mammalian cells due to toxic effects. Here, the authors report on a self-assembled nano-ballistic delivery system for the delivery of functional macromolecules and demonstrate efficient transfection of mammalian cells.
- Subjects
MACROMOLECULES; ELECTROPORATION; PULSED lasers; POISONS; GENE transfection
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-29713-7