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- Title
Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure.
- Authors
Kremplova, Monika; Fialova, Dana; Nejdl, Lukas; Hynek, David; Trnkova, Libuse; Hubalek, Jaromir; Kizek, Rene; Adam, Vojtech
- Abstract
The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected-peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.
- Subjects
ADENINE nucleotides; PYRIMIDINES; SQUARE waves; VOLTAMMETRY
- Publication
Materials (1996-1944), 2014, Vol 7, Issue 3, p1455
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma7031455