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- Title
ZAK negatively regulates RhoGDIβ-induced Rac1-mediated hypertrophic growth and cell migration.
- Authors
Chih-Yang Huang; Li-Chiu Yang; Kuan-Yu Liu; I-Chang Chang; Pao-Hsin Liao; Janet Ing-Yuh Chou; Ming-Yung Chou; Wei-Wen Lin; Jaw-Ji Yang
- Abstract
RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIβ plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIβ expression by RNA interference blocked RhoGDIβ-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDIβ in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDIβ.
- Subjects
CYTOLOGY; CELL migration; CELL lines; CELL culture; GENETIC transcription; GENETIC code
- Publication
Journal of Biomedical Science, 2009, Vol 16, p1
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1186/1423-0127-16-56