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- Title
In vivo protein crystallization opens new routes in structural biology.
- Authors
Koopmann, Rudolf; Cupelli, Karolina; Redecke, Lars; Nass, Karol; DePonte, Daniel P; White, Thomas A; Stellato, Francesco; Rehders, Dirk; Liang, Mengning; Andreasson, Jakob; Aquila, Andrew; Bajt, Sasa; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J; Bostedt, Christoph; Boutet, Sébastien; Bozek, John D; Caleman, Carl; Coppola, Nicola
- Abstract
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
- Subjects
TRYPANOSOMA brucei; SYSTEMS biology; CRYSTALLOGRAPHY; X-ray diffraction; FREE electron lasers; PROTEINS
- Publication
Nature Methods, 2012, Vol 9, Issue 3, p259
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1859