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- Title
Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors.
- Authors
Netea, Mihai G.; Gow, Neil A. R.; Munro, Carol A.; Bates, Steven; Collins, Claire; Ferwerda, Gerben; Hobson, Richard P.; Bertram, Gwyneth; Hughes, H. Bleddyn; Jansen, Trees; Jacobs, Liesbeth; Buurman, Ed T.; Gijzen, Karlijn; Williams, David L.; Torensma, Ruurd; McKinnon, Alistair; MacCallum, Donna M.; Odds, Frank C.; Van der Meer, Jos W. M.; Brown, Alistair J. P.
- Abstract
The fungal pathogen Candida albicans has a multilayered cell wall composed of an outer layer of proteins glycosylated with N- or O-linked mannosyl residues and an inner skeletal layer of beta-glucans and chitin. We demonstrate that cytokine production by human mononuclear cells or murine macrophages was markedly reduced when stimulated by C. albicans mutants defective in mannosylation. Recognition of mannosyl residues was mediated by mannose receptor binding to N-linked mannosyl residues and by TLR4 binding to O-linked mannosyl residues. Residual cytokine production was mediated by recognition of beta-glucan by the dectin-1/TLR2 receptor complex. C. albicans mutants with a cell wall defective in mannosyl residues were less virulent in experimental disseminated candidiasis and elicited reduced cytokine production in vivo. We concluded that recognition of C. albicans by monocytes/macrophages is mediated by 3 recognition systems of differing importance, each of which senses specific layers of the C. albicans cell wall.
- Subjects
CANDIDA albicans; CELLULAR immunity; CYTOKINES; MACROPHAGES; POLYSACCHARIDES; LEUCOCYTES; CELL metabolism; ANIMAL experimentation; CANDIDIASIS; CELL receptors; CELLS; COMPARATIVE studies; DOCUMENTATION; GLUCANS; RESEARCH methodology; MEDICAL cooperation; MICE; MOLECULAR structure; RESEARCH; EVALUATION research; MONONUCLEAR leukocytes
- Publication
Journal of Clinical Investigation, 2006, Vol 116, Issue 6, p1642
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI27114