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- Title
At5g54160 gene encodes Arabidopsis thaliana 5-hydroxyconiferaldehyde O -methyltransferase.
- Authors
Tomoyuki Nakatsubo; Yu Kitamura; Norikazu Sakakibara; Masaharu Mizutani; Takefumi Hattori; Nozomu Sakurai; Daisuke Shibata; Shiro Suzuki
- Abstract
Abstract The function of an Arabidopsis thaliana gene, At5g54160 annotated as a caffeic acid O-methyltransferase CAOMT gene was characterized. The recombinant enzyme of this gene (AtOMT1) catalyzed the O-methylation of phenylpropanoid and flavonoid substrates. The specificity constants (k cat/K m) for 5-hydroxyconiferaldehyde (5-HCAld) and quercetin were both 0.11 μM−1·min−1. On the other hand, lignins of At5g54160-knockout Arabidopsis mutants lacked syringyl units. In addition, we showed that the gene silencing also resulted in significant accumulation of caffeyl alcohol (CaAlc). These results strongly suggested that At5g54160 gene is involved in syringyl lignin synthesis for the methylation of both 5-hydroxyconiferaldehyde and 3,4-dihydroxyphenyl compound(s).
- Subjects
ARABIDOPSIS thaliana; GENE expression; METHYLATION; LIGNINS; ALCOHOL; GENETIC regulation
- Publication
Journal of Wood Science, 2008, Vol 54, Issue 4, p312
- ISSN
1435-0211
- Publication type
Article
- DOI
10.1007/s10086-008-0958-4