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- Title
Fungal community structure at pelagic and littoral sites in Lake Biwa determined with high-throughput sequencing.
- Authors
Song, Peixue; Tanabe, Shoko; Yi, Rong; Kagami, Maiko; Liu, Xin; Ban, Syuhei
- Abstract
Fungi may play an important role in material cycling in lakes and oceans; however, only limited information is available on fungal community structure, especially in large lakes such as Lake Biwa. In this study, whole fungal communities were determined seasonally and spatially using a high-throughput sequencing technique. Water samples were collected from the epilimnion, 0-20 m depth, with a Van Dorn sampler at a pelagic site and from the surface at a littoral site in the north basin of Lake Biwa. All pelagic depth samples were combined into one sample. Sampling occurred on 24 April, 22 May, 10 July, and 16 September 2015. DNA was extracted from filtered samples. Metabarcoding analysis targeting fungi-specific internal transcribed spacer 2 regions was performed using an Illumina MiSeq platform. Epilimnetic fungal communities showed high diversity, with 479 operational taxonomic units (OTUs). The OTUs included 122 belonging to the phylum Ascomycota, 127 to Basidiomycota, 38 to Zygomycota, 45 to Chytridiomycota, 2 to Glomeromycota, and 145 were unclassified fungi. Fungal community structures varied seasonally and spatially. Few of the fungal OTUs overlapped between seasons and sites, and specific communities of fungi were detected on each sampling occasion. Results indicated that spatio-temporal variations in fungal communities were high and may be influenced by both internal factors and external factors, such as terrestrial inputs.
- Subjects
BIWA Lake (Japan); FUNGAL communities; LITTORAL zone; SPATIO-temporal variation; NUCLEOTIDE sequencing
- Publication
Limnology, 2018, Vol 19, Issue 2, p241
- ISSN
1439-8621
- Publication type
Article
- DOI
10.1007/s10201-017-0537-8